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Training mice at glucocorticoid peaks resulted in higher rate of spine formation, whereas glucocorticoid troughs following training was necessary for stabilization of spines formed during training and long-term memory retention (Liston et al., 2013)

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Bioinformatic analyses Structural models were retrieved from the AlphaFold Protein Structure database 75,76 or predicted using the ColabFold software 77

P2 was suspended in 10 vol of 0.32 M sucrose and 10 ml of the solution layered on a 0.8 and 1.2 M two-step discontinuous gradient, which was centrifuged at 61,000 x g for 2 h to separate the synaptosomes from the mitochondria (which forms the pellet)

R.EisenA.HardimanO.et al
