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After incubation, to remove excess DCFH-DA, the stained bacteria were centrifuged at 1,000 g for 5 min and resuspended in fresh M9 medium to OD 600 = 0.5

The injections do not involve any specific preparation instructions

Researchers hypothesize that caffeine's ability to block receptors in the brain that facilitate the growth of beta-amyloid plaques (a pathologic hallmark of Alzheimers) could be one mechanism behind this protective effect