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The rings were mounted in an isolated organ chamber containing Krebs solution (pH 7.4) at 37 C and kept constant aerating with 95% O 2 /5% CO 2

Choline is an essential nutrient for humans as it helps give cell membranes structural strength, provides structure to cell membranes, is involved in the signaling across this membrane, as well as being involved in the synthesis and release of the neurotransmitter acetylcholine

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Primary endpointhospitalization within 21 days One patient (2%) in the RV group and 3 (6%) in the placebo group (Table 2) were hospitalized within 21 days of symptom onset (risk ratio (RR) = 0.33

Western blots and antibodies Protein samples were separated by SDS-PAGE and proteins in the gel were transferred onto PVDF membranes, followed by blocking with non-fat milk for 1 h at room temperature and incubation with specific primary antibodies (diluted at 1:1000 to 1:5000) overnight at 4 C