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Rodriguez-Muela N, Germain F, Marino G, Fitze PS, Boya P

mRNA was enriched with oligo-(dT) beads, and subsequently, cDNA was generated with poly (A) added to the 3 end

The soluble protein-containing fraction was separated by centrifugation at 24,650 g for 20 min at 4 C and purified by Ni 2+ affinity chromatography with the use of TALON His-tag

Mol Asp Med 63:8898 Hempel N, Carrico PM, Melendez JA (2011) Manganese superoxide dismutase (Sod2) and redox-control of signaling events that drive metastasis

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