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By simple adjustment in solution pH from mild to highly basic, they managed to selectively quantify GSH and GSSG by using fluorometric detection at 340/425 nm

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he low pH (3.6) required for the assay may cause protein precipitation (e.g., casein in milk samples) or other artifacts

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To avoid a potential risk of hypercobalaminemia or possible health risks of high-dose dietary supplements, an annual blood test is recommended, after which targeted substitution can be performed

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