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4c, Extended Data Fig

Mutations in Parkin or PINK1 impair these proteins, leading to the accumulation of damaged mitochondria, reduced energy production, and elevated levels of reactive oxygen species (ROS) 39

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Isolation of rat heart mitochondria Upon cervical dislocation of 912 weeks old male Wistar rats (Charles River, UK), heart mitochondria (RHM) were prepared by homogenization of their heart tissue in STEB buffer (250 mM sucrose, 5 mM Tris-HCl and 1 mM EGTA, pH 7.4) supplemented with 0.1% fatty acidfree BSA

10.1111/nan.12919 292 YaoX.ZhangY.HaoJ.DuanH