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More patients have been diagnosed since the inclusion of lcFAODs in NBS panels, often before development of symptoms and it is debated whether dietary restrictions should be alleviated in certain patient groups [147, 148]

To test this hypothesis, they analyzed the lipid composition of GPMVs taken from cells grown at 20 or 28 C using a shotgun lipidomics technology platform

Cholesterol ( m/z = 386.3), 7-dehydrocholesterol ( m/z = 384.3) and 4-methyl-5-cholest-8(14)-en-3-ol/lophenol ( m/z = 400.3) were identified by comparison of elution profiles and fragmentation patterns with reference to the NIST database and cholesterol as well as 7-dehydrocholesterol standards
