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After washing with 1 ml PBS solution, cells were further crosslinked with 3 mM disuccinimidyl glutarate (Fisher Scientific) in 2.5 ml PBS solution at 30 C for 40 min
Preclinical and some early clinical research has examined whether replenishment strategies affect neurological outcomes
Gopal VK, Francis SH, Corbin JD
& Provenza, F
Effect of silymarinon on chemical, functional, and morphological alterations of the liver: a double blind controlled study
