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BioLegend, 305104, clone G10F5) were analyzed by flow cytometry, as described previously 40 , using a NovoCyte 3000 flow cytometer with BD FACS Diva 6 software

In the first step, enzymes such as cytochrome P450 monooxygenases catalyze various reactions (oxidation, reduction or hydrolysis) to expose or introduce a functional moiety on hydrophobic substrates

Samples were prepared in the presence of (i) 1 mM phosphate buffer (pH 7.5), (ii) 1 mM phosphate buffer (pH 7.5) with 5% TFA (v/v), and (iii) 1 mM phosphate buffer (pH 7.5) with 1 mM TCEP

Larger studies have looked at genetic loci linked to hyperuricemia, as compared to gout, which optimally requires robust clinical validation of the diagnosis

PMCID: PMC11365797