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siRNAs containing 5-fluoro-2-deoxyuridine (FdU) moieties can suppress targeted gene expression

The majority of EGFP+ cells with activated NF-B signaling represent IBA1+ macrophage/microglia cells, followed by astrocytes, with NG2-glia acting as minor contributors at day 3 and 7 post-TBI (Fig

Once all samples have been collected, they were stored in a -80 C ultra-low temperature freezer until use for determining the relative expression levels of the NtGSTU10 gene, as well as for transcriptomics sequencing and metabolomics analysis
doi: 10.1016/j.cmet.2013.08.005 245 SanyalACharlesEDNeuschwander-TetriBALoombaRHarrisonSAAbdelmalekMFet al