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Notably, some ferroptosis inhibitors such as iron chelators, Fer-1, Lip-1, ROSI (ACSL4 inhibitor), and Trolox (antioxidant vitamin E analog) have been demonstrated to significantly inhibit ferroptosis in NAFLD animal models induced by arsenic, CDE-fed, MCD-fed, HFD-fed, and so on, and attenuate liver inflammation reactions, lipid accumulation, and liver injury [134,135,136,137,138]

As a positive control for apoptosis, tamoxifen (50 M) was included ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells

Extracellular Matrix and Collagen Studies GHK-Cu is frequently used in studies involving collagen production, fibroblast activity, and extracellular matrix remodeling

doi: 10.3390/ijms21249639 301 FongLKYangMMDos Santos ChavesRReynaSMLangnessVFWoodruffGet al