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TGF-1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency

After filtration through miracloth (Calbiochem) and clarification by centrifugation (15,000 g , 20 min, 4C), the Strep -GSTs were affinity purified as described ( in vitro pull-downs were analyzed by HPLC-MS as described ( Results Fishing for Ligands of Plant GSTs in planta : Transient Expression in N

Stability depends on formulation, handling, and storage conditions rather than format alone

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