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Then, 1 mL of the overnight culture was transferred into 50 mL of LB or M9 medium in 250 mL flasks for further cultivation (kanamycin or ampicillin was added when needed)

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High-dose methionine supplementation has theoretical concerns regarding homocysteine elevation, though this is unlikely at typical lipotropic injection doses

5h), suggesting that the observed drop was due to lipid oxidation (Fig