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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Protein refolding Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating

This Primer by Brissot and colleagues discusses the aetiology, epidemiology and diagnosis of haemochromatosis and describes patient management and quality of life.

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