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Twenty-four hours after low glucose treatment, the media was replaced by DMEM containing 2 5mM glucose, and recombinant human insulin was added to a final concentration of 25 M

References Newborn Reflexes and Behavior

I realize that the way something looks doesn't necessarily indicate how well it works, which is the case here.~~ The buttons are very loud and 'clicky' when pushed, But, maybe this will help them last longer

Equal numbers of eggs (~ 800) were loaded per lane

This will ensure that the LiPO is fully discharged to 0V
