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The substantial alterations in the levels of differential metabolites observed between the blank group and the treated group suggest that the metabolic pathways have been profoundly impacted (Fig

The results were analyzed using the 2 CT method with gapdh as a reference gene (Livak and Schmittgen, 2001)

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DNA Damage Oxidative stress in cells can generate DNA strand breaks and base modifications, most notably 8-hydroxy-2-deoxyguanosine (8-OHdG), a widely used marker of oxidative DNA damage in research settings

The goodness of t was estimated using the coefcients of determination (r 2 )