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In this study, we measured tail flick latency (TFL), arterial blood gas (ABG) chemistry, Alveolar-arterial gradient, and ventilatory parameters by whole body plethysmography to determine the responses elicited by bolus injections of fentanyl (75 g/kg, IV) in male adult SpragueDawley rats that had received a bolus injection of GSHee (100 mol/kg, IV) 15 min previously
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Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GSTo1
