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Ten larvae from each control and treatment group were added to 1 mL of acridine orange staining solution with a final concentration of 5 g/mL, incubated at room temperature for 30 min, washed three times with E3 medium, and excited with 488 nm laser under a fluorescence microscope to observe and photograph the fluorescence of the larvae

Marquard J, Otter S, Welters A, Stirban A, Fischer A, Eglinger J, et al
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Because there is such a diverse range of initial symptoms, vitamin B12 deficiency often goes unrecognised

A meta-analysis of 39 prospective cohort and case-control studies found dietary folate intake to be inversely associated with risk of breast cancer, but data stratification revealed the association was evident in premenopausal women and not in postmenopausal women (144)
