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(2019) The association between mushroom consumption and mild cognitive impairment: A community-based cross-sectional study in Singapore

The cell lysate was precipitated out using acetone and centrifuge at 1500 x g for 10 min at 4 C

In the last step, a second GSH is used to regenerate the enzyme and form glutathione disulfide (GSSG) (Savaskan et al., 2007)

For analysis of disulfide bond formation by MALDI, the samples were desalted and concentrated using C18 Ziptip pipette tips (Millipore)

In contrast, a clinical trial specifically testing N-acetylcysteine in 43 patients with probable Alzheimers disease reported that N-acetylcysteine (50 mg/kg/day) for 6 months failed to significantly alter MMSE scores, which were the primary outcome measures (18)