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Simple in vitro generation of human leukocyte antigen-Gexpressing T-regulatory cells through pharmacological hypomethylation for adoptive cellular immunotherapy against graft-versus-host disease

After centrifugation (1,500 g for 3 min), the cell pellet was gently resuspended in Dulbeccos modified Eagle medium (Invitrogen, San Diego, CA, USA) containing 10% fetal bovine serum, 4 mM L-glutamine, and 60 mg/l kanamycin sulfate (growth medium)

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