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The cells or tissue sections were fixed in 4% paraformaldehyde in PBS for 15 min, and permeabilized with 0.1% Triton X-100 (Sigma-Aldrich) and blocked with 5% goat serum in PBS and then incubated with anti--catenin (1:100

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In normoxia (normal oxygen levels), prolyl hydroxylase hydroxylates HIF-1 at two proline residues (Pro 402 and Pro 564)

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