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Immunostaining intensity was assessed quantitatively by immunohistomorphometry using ImageJ software (National Institutes of Health, Bethesda, MD), as described previously[48]

In people affected by prevalent chronic inflammatory dermatoses like psoriasis or eczema, GLUT1 expression is increased in proliferating keratinocytes [4] and is significantly upregulated in hypertrophic scars [31]

Yes , for any in vitro biological assay where the reconstituted peptide is added to cell cultures or tissue preparations, matched Bacteriostatic Water vehicle controls at the same final benzyl alcohol concentration as the highest peptide treatment concentration should be included to confirm absence of benzyl alcohol-mediated biological effects on the assay endpoints measured

Proc Natl Acad Sci USA 97(24):1335413359