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Conjugated bilirubin can be measured in the same reaction by omitting the solubilizing agent (since it is already in a conformation where the diazo dye can react this is because conjugation itself disrupts those hydrogen bonds)

3.2 Pharmacodynamic Interactions The key mechanisms of action of anti-cancer drugs involve apoptosis, autophagy, and cell cycle arrest (Wang et el., 2011

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Cancer Res 82:CT108CT108 Schuhmacher J, Heidu S, Balchen T, Richardson JR, Schmeltz C, Sonne J, Schweiker J, Rammensee HG, Thor Straten P et al (2020) Vaccination against RhoC induces long-lasting immune responses in patients with prostate cancer: results from a phase I/II clinical trial