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Equal protein loads were analyzed by western blotting using: pSTAT3 (Tyr705), STAT3, or GAPDH loading control (Cell Signaling)

This pioneering work was published in the journal Science [5].NETs are extracellular fibrous structures composed of a DNA scaffold along with histones and neutrophil-derived granular enzymes, such as neutrophil elastase (NE) and myeloperoxidase (MPO), among other related proteins (Figure 1)

Additionally, we found that HT treatment effectively prevented the decline in protein expression of Occludin and Claudin1 in IPEC-J2 cells, and ML385 counteracted these protective effects (Fig

Overall, this review should be viewed as hypothesis-generating, mapping candidate pathways and highlighting priorities for well-designed intervention studies to test these mechanisms and strengthen causal inference
