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[82] The French term was first used in 1880 by Jean-Baptiste-douard Glineau, who used the Greek ( nark ), meaning "numbness", and ( lepsis ) meaning "attack"

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Equal amounts of total protein were separated by SDSPAGE, transferred to a PVDF membrane using either the Trans-Blot Turbo System (#170-4273, BIO-RAD) following the manufacturers protocol or tank blotting in 2.5 mM TRIS base, 20 mM glycine, 15% methanol

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Over a thirty-five year course from the year 1980, the number of individuals with DM increased from one hundred eight million to over four hundred twenty-two million individuals ( 1c ) parameters ( Metabolic disease affects low and middle income countries more than high income developed countries with approximately eighty percent of people residing in low-income nations (54)

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