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Another point of interest regarding B12 supplementation is the commonly held belief that intramuscularinjections of B12 are more effective than oral supplementation

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For instance, the pKa of the SH group decreases (usually to 5.0-7.0) in an immediate vicinity of basic amino acid residues (His, Lys, and Arg), whereas at physiological pH, the sulfhydryl groups dissociate

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The platelet surface fluorescence intensity was analyzed using a FacsCanto II flow cytometer (BD Biosciences, San Diego, CA, United States) to analyze the expression of platelet surface membrane proteins