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SDS-PAGE was run in a PROTEAN II XL cell (Bio-Rad) at 75 mA/gel

This intertwined complexity in both machinery and assembly is not conducive to traditional genetic approaches to either label components or utilize knockout technologies to identify regulatory pathways

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IGF-1 LR3 (Long R3 Insulin-Like Growth Factor-1) is a synthetic analogue of human IGF-1 , engineered for enhanced stability and biological activity in research settings

The integration of these natural and food-derived compounds represents a promising strategy for mitigating neuroinflammation, reducing oxidative stress, and promoting post-stroke recovery (Figure 3)