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Cells were washed three times with RPMI1640 medium and incubated with 1 g ml 1 Alexa Fluor 594 goat anti-rat IgG (HL) for 5 min at 4 C
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Hypertrophic and keloid scars fail to progress from the CD34 - /-smooth muscle actin (-SMA) + immature scar phenotype and show gradient differences in -SMA and p16 expression