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Next day, HEK293T cells were transfected with the DNA constructs of Venus-tagged mini Gs (2000 g/well), full-length CTR that was C-terminally tagged with NanoLuc luciferase (100 g/well), and one type of full-length RAMPs (100 g/well) for 2 days at 37, 5% CO 2

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PMID: 10469335 Goldstein AL, et al

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