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They were provided ad libitum access to standard rodent chow and water

Preservative-free formulations, often requested for sensitive individuals, almost always require refrigeration

Beads were washed with low salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 150 mM NaCl), high salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 500 mM NaCl), LiCl buffer (250 mM LiCl, 1% NP40, 1% NaDOC, 1 mM EDTA, and 10 mM TrisHCl, pH 8), twice with TE buffer(10 mM TrisHCl, pH 8, and 1 mM EDTA), and finally with elution buffer (1% SDS and 100 mM NaHCO 3 )

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Figure 4 In summary, melatonin plays a pivotal role in regulating female reproductive hormone secretion and the menstrual cycle rhythm through its complex interactions with the hypothalamic-pituitary-ovarian (HPO) axisEnvironmental light exposure, patterns of melatonin secretion, and the dynamics of receptor expression collectively contribute to the maintenance of endocrine balance and physiological functions within the female reproductive system (78, 79)
