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The MnSOD gene polymorphism (Val16Ala, also referred to as Val9Ala in some studies) results in an Ala variant at position 9 in the mature protein (position 16 in the precursor protein) that increases the efficiency at which MnSOD is transported into the mitochondria, theoretically promoting hydrogen peroxide generation in this organelle (381)

So, if you know how much of your GST-fusion protein youre going to load onto the beads, it will be very easy to calculate the volume of beads that you need

[DOI] [PMC free article] [PubMed] [Google Scholar] Bucio L

But the clinical question isnt Can you stack peptides? Its: Does the combination make biological sense, does it have human evidence, and can you monitor it safely enough to justify the added complexity

Hydration: Keep fluids up across the protocol