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Salman MM, Zaki AM, El-Gamal HH, Gomaa MF, Osman AA, Farid LA
Regulation of endoplasmic reticulum turnover by selective autophagy
The amplified sequences were inserted into the suicide vector pSDUDI after digestion with appropriate restriction enzymes
The glutathione resins selectively bind to GST-tagged proteins effectively, allowing the specific protein of interest be separated from the mixture at high efficiency
Notably, relatively small alterations in Ca 2+ related to depolarization of mitochondria are insufficient to affect neurotransmitter release via normal physiological mechanisms
