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The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker
GHK-Cu works through regenerative pathways including collagen production, growth factor secretion (VEGF, HGF), and follicle enlargement
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[DOI] [PubMed] [Google Scholar] Chen W., Abramowitz M.K
