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The strain overexpressing GSH1/GLR1 produced 14.0 g L-1 ethanol in 48 hours corresponding to an ethanol yield on hexoses of 0.17 g g-1

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Nickel affinity chromatography The nickel on agarose beads binds to proteins through polyhistidine tags, known as his-tags, that usually range from 6 to 10 histidine residues long (Figure 2)

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