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First, the RNA was heated at 95 C for 5 min in 1 PKN buffer and then cooled to 4 C to allow folding

10.1016/s0140-6736(14)61838-61839 Lancet 142 WuH.TaoJ.LiX.ZhangT.ZhaoL.WangY.et al (2017)

Wessels I, Maywald M, Rink L (2017) Zinc as a gatekeeper of immune function

Biochemistry 30 , 4247 (1991)

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