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The next day, the samples were each washed twice with Wash Buffer 1 (20 mM Tris-HCl pH 7.5, 1% Triton X-100, 2% SDS, 100 mM NaCl, supplemented with protease and phosphatase inhibitors) and then with Wash Buffer 2 (20 mM Tris-HCl pH 7.5, 0.5% Triton X-100, 100 mM NaCl, supplemented with protease and phosphatase inhibitors) and finally washed twice with Wash Buffer 3 (20 mM Tris-HCl pH 7.5 and 100 mM NaCl)

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(2024) Breast Cancer Res, 26: 179