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Membranes were extensively washed in PBS containing 0.1% vol/vol Tween20 before incubation with anti-mouse (# 115-035-003) and/or anti-rabbit (# 111-035-144) IgG secondary antibodies 1:3000 (Jackson ImmunoResearch, Cambridge, UK) for 90 min at RT

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3C, hydrogel was very stable in aqueous solution without H 2 O 2 and no significant shape change was observed up to 960 h (40 days) incubation

Legally compounded access generally requires a licensed-prescriber evaluation and a valid patient-specific prescription, with the preparation made by a 503A pharmacy or 503B outsourcing facility but only if the ingredient is eligible under the bulk-substance rules [20]

Machine learning algorithms further facilitate automated quantification of cardiac parameters, detection of cardiovascular pathologies, and assessment of cardiac function, which improves the diagnostic accuracy and efficiency for diseases (116, 117)