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5.1 Probiotics and intestinal barrier protection The intestinal epithelium functions as a critical first line of defense, preserving epithelial integrity and shielding the host from pathogenic microorganisms (158)

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The culture medium was aspirated after 24 h and the dishes were washed with PBS

High-throughput in vitro drug and combination screening assay A high-throughput in vitro drug screening assay was used to quantify the chemosensitivity of leukemia cell lines as described previously ( 2 and 37C), each well was filled with 73 l of culture media and left overnight in the incubator for cell acclimatization

Using samples from 680 patients almost 20 years later, these researchers found that 5% were B12 deficient on entering the study