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These mechanisms of action are consistent with the elevated liver PHGDH and PSAT1 mRNA and protein in response to SAAR

doi: 10.1186/s12866-025-04072-7

Briefly, frozen pellets were resuspended with Milli-Q water and homogenized using a Pellet Pestle Motor (Kimble) for 30 s, alternating with 10 min incubation on ice (repeated for three times)

Rev Nutr

Purified R001 was dissolved in 100% DMSO for a stock solution of 50 mM (stored at 20 C), which was freshly diluted down to a series of working solutions with culture medium or phosphate-buffered saline (PBS) and used to achieve the final concentrations as noted in the manuscript