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Tip: Taking your injection with food can help reduce stomach upset
Everything was very clean and sterile
The 2-hour SDS treatment was continued every other day, with 2 M svL4 in PBS alone or mixed with 1 M Dex sodium phosphate (catalog number 2480645, Henry Schein, Melville, NY) in 0.2 ml of PBS applied daily for another 5 days
Ice crystal formation disrupts the peptides structure, and repeated freeze-thaw cycles have been observed to reduce biological activity by 20-50% (2)
The animals tendency to avoid the center area reflects the anxiety related behavioral change 27
