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The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board
Li L, Tan J, Miao Y, Lei P, Zhang Q

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DI Keller J Grenier G Christ 2009 Characterization of novel KCNH2 mutations in type 2 long QT syndrome manifesting as seizures Can J Cardiol 25(8):455-462 Kathmann M, Flau K, Redmer A, Trnkle C, Schlicker E

Safety and tolerability of injectable lipid-lowering drugs: a review of available clinical data
