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Then, the quantity of RNA was determined by NanoDrop (Aosheng, China) and 1 g of total RNA was transcribed to complementary DNA (cDNA) through 1 L of random hexamer primer
The results showed that liraglutide had a modest positive effect on improving motor function and performed well in terms of safety and tolerability, although there were some manageable gastrointestinal side effects

R&D Systems, Inc., Minneapolis, MN), according to the manufacturers protocol

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After two weeks of AAV vector exposure, we confirmed a significant reduction in VGF signal in the -cells of GCGR-KO mice injected with AAV-sh-VGF (Fig
