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The sample blank is not affected because the calibration curve is created based on the slope of the calibration curve

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Of note, that BPC 157 effect is particular (i.e., activated the phosphorylation of VEGFR2, Akt, and eNOS signaling pathway) and does not need other known ligands or shear stress (Hsieh et al., 2017)

Although the exact mechanism of interstitial cystitis is unknown, there is thought to be a significant contribution from chronic inflammation, therefore BPC-157 was chosen in this study due to its anti-inflammatory properties and its role in restoring damaged epithelium as demonstrated in various animal models [22, 66, 67]