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Inter-organelle membrane contact sites (MCSs) are physical contacts formed between two membrane-bound organelles held together by tethering forces, characterized by a well-defined lipidome and proteome while serving a specific biological function such as molecular transport or signaling (118)

The sam1/sam1 mutant cells likely exhibited decreased growth in this condition due to downregulated CDC21 expression (fold change of 0.62, p-value=0.0247), which encodes thymidylate synthase, and/or because RNR complex inhibition could create dNTP pool imbalances which in concert with less Cdc21 could cause more difficulty to interconvert between some deoxyribonucleotides in these mutant cells, thereby creating sensitivity to this condition (Fig 13)

All lines were tested negative for mycoplasma

Journal of Neurosurgery , 2011