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Double Immunolabeling Following first primary antibody, tissue was subsequently blocked in 5% normal horse serum, followed with separate blocking steps in streptavidin and biotin solutions (from ABC kit) five minutes each before second primary antibody reaction
This group primarily encompasses a range of rare and complex conditions, including multiple sclerosis, autoimmune encephalitis, neuromyelitis optica spectrum disorders, autoimmune epilepsy, myasthenia gravis, and central nervous system demyelinating diseases, among others, and affects individuals across a wide age range [170]

[DOI] [PMC free article] [PubMed] [Google Scholar] 145.Li, P

Moreover, there was a direct relationship between serum levels of IGF-1 and the skeletal parameters relating to bone size
Chem Phys Letters 478:266270
