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The concentrations and yields of GSH based on l-cysteine for these engineered strains are shown in Fig

The liver tissue was homogenized with a buffer solution having 250 mM sucrose, 50 mM Tris-HCl (pH 7.4), and 1 mM EDTA and stored with 4% paraformaldehyde (PFA) or at 80C

The lifespan-extending effects of MR have been attributed to a number of different mechanisms, including MR-induced reductions in oxidative stress [30] and inflammation [23], alterations in autophagy [37], and increases in cardioprotective hormones [15,38,39]

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