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The culture medium was Dulbecco's Modified Eagle Medium (DMEM, D5796), supplemented with 10% fetal bovine serum (FBS, F7524), and 100 U/mL Penicillin-Streptomycin (P4333), referred to as complete DMEM. For HT29-MTX cells, 1% MEM Non-essential Amino Acid Solution (M7145,) was also added