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The reaction mixture was incubated for 1.5 h at 37 C and then heated with SDS loading buffer and analyzed using SDS-PAGE electrophoresis and immunoblotting

These in-office procedures are typically followed up with relative rest and physical therapy

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Studies also reported modulation of pain signaling through both peripheral and dopaminergic mechanisms

10.1016/j.neuint.2014.02.008 Neurochem Int
