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Description
Cells were washed three times with 1 ml PBS and lysed with 200 l 0.5% (v/v) Triton X-100 (Fisher Scientific) at 37 C for 5 min, followed by the addition of 800 l PBS

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Reconstitution errors Using the wrong diluent ranks among the most serious errors

Supports physiologic IGF-1 production downstream of GH signaling

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