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This is done at a temperature range of 55 to 65 or under reduced pressure conditions
The TaqMan Gene Expression Assay (Applied Biosystems, Termo Fisher Scientific, Waltham, MA, USA) with the TaqMan Gene Expression Master Mix were used for the gene expression analysis of selected genes (Table 1)
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The peptide is unaffected dilution changes the volume per dose, not the compound
the resin was transferred to a sintered glass funnel (4 porosity) and the DMF removed by vacuum
